Daily Maintenance| 79
Daily Maintenance
Daily maintenance involves flushing the washer with an appropriate reagent or
deionized water throughout the day. Routine rinsing helps to prevent the aspirate
and dispense tubes from clogging between runs. Flushing the devices with deionized
water is recommended at the end of the day for most applications.
The recommended rinsing frequency depends on the solutions currently in use:
l When a solution containing surfactant is used throughout the day, perform the
rinsing procedure when the device is idle for more than 3 hours.
l When the solution does not contain surfactant, consider rinsing at least once an
hour.
Run this protocol and enable AutoPrime to satisfy the daily maintenance
requirements:
l W-DAY_RINSE
Make sure the supply bottles contain sufficient rinse solution and that the waste
bottles are empty before running the protocols.
Also see the additional maintenance procedures required when dispensing protein
solutions: Removing Protein Residuals and Fungi Growth on page 82.
AutoPrime
AutoPrime automatically conditions the dispense tubes, priming them with the
specified volume, after a user-specified amount of idle time.
l Press the STOP button to interrupt the AutoPrime routine when it is underway.
l Any interaction with the instrument via the touch screen or the LHC resets the
interval clock.
Overnight/Multi-Day Maintenance
Overnight/multi-day maintenance involves flushing all solutions out of the
instrument, and then periodically rinsing and soaking the tubes to keep them moist.
Here are three recommendations for accomplishing the task. Employ the method that
best suits your work flow:
BioTek Instruments, Inc.
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