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Gatan ORIUS SC 200D - Dark Reference Image Calibration

Gatan ORIUS SC 200D
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ORIUS ™ 833 User’s Guide Series Rev 3 2-3
Figure 2-2 (a) Camera Menu (b) Camera configuration
Select Configure Camera in the Camera menu (Figure (2-2a).
In the Camera configuration dialog box (Figure 2-2b), you can change
the name of the camera by typing in the Name field.
Image rotation can be specified in the Rotation pull-down menu.
The shutter state should only be setup IF the camera was configured
with the optional shutter for this camera
2.3 Dark Correction
After the camera has cooled to its operating temperature of 10°C, a
calibration of the bias or thermal counts is required. This is often
referred to as the “dark image”.
Quick tip: A dark reference image set must be taken before preparing a
gain reference.
To prepare a dark reference image obscure the camera, either by
lowering the TEM screen, retracting the camera or turning off the
TEM beam.
Under the camera menu select “Prepare dark reference”.
Wait about 15 minutes for the procedure to complete.
It should not be necessary to repeat this more that once per week and
may be good indefinitely.

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