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Gatan ORIUS SC 200D - Frequently Asked Questions; Troubleshooting Common Camera Issues

Gatan ORIUS SC 200D
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ORIUS ™ 833 User’s Guide Series Rev 3 4-3
It is OK to run the Peltier cooler in the warm position overnight,
but do not run it when the camera is at air pressure or if the cooling
water is not flowing.
Check vacuum system.
If oil accumulates even with regular warming of the scintillator, it
is recommended that the vacuum system be checked. If no leak
exists then installing a foreline trap on the rotary pump is
recommended.
Examine the surface of the scintillator.
If the surface of the scintillator becomes dirty or scratched, ship the
camera to Gatan for service. Do not attempt to clean the scintillator
yourself as damage can result.
4.2 Most Often Asked Questions
The following are some of the questions most frequently asked by camera users. If you are having a problem
with your camera, check and see if the tips listed below are helpful.
1. There is no image on the CCD, what should I do?
Check the “Camera View” floating window to see if the “Camera Inserted”
box is checked (camera inserted).
Check the camera really inserted and is not stuck out. With the ORIUS™
camera you can see the can move in and out.
Check if the beam has drifted.
Check if the TEM viewing screen is lifted.
Check if you have a film plate stuck above the camera in the TEM.
You may have an image and not being able to see it. Move the pointer
around to get an idea of the counts. If the value is over 16,000 counts (in
1x binning mode) the CCD is saturated. Reduce the beam intensity or
shorten the exposure time.
2. How often do I have to acquire a gain reference?
Whenever the image quality seems to be deteriorating. The most reliable
way to see if a new gain reference is advisable is to take a gain normalized
image of uniform illumination with no specimen in the beam and look for
any spurious pattern. If you see one, update the gain reference.
3. What is the shortest and/or the longest exposure time I can
use?

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