ORIUS ™ 833 User’s Guide Series Rev 3 2-5
2.5 Magnification Calibration
The displayed nominal magnification on TEM is for photographic film and has an accuracy of 5-10%. The CCD
camera is located on a different plane (height wise) respect to the film camera. As a consequence, the
magnification must be calibrated. The calibration is done using standard calibration samples.
At “Low” magnifications:
• Use “cross grating sample” or any sample having known spacing
At “High” magnifications:
• Use “graphite” or any crystalline samples having known lattice
spacing
• Use the FFT method.
It is very important to make sure DigitalMicrograph software correctly reads the TEM magnification. If the
communication between the computer and the TEM is established, the magnification is read automatically.
Otherwise, please make sure DigitalMicrograph is set to prompt the user to enter a value for TEM magnification
every time an image is to be acquired. This can be set by choosing the “Global Microscope Info” window
shown in Figure 2-4 under the Microscope menu (Figure 2-5).
Figure 2-4 Global Microscope Info window
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