GE AKTA avant - Before You Prepare the System

5.1 Before you prepare the system
Introduction
It is important to prepare the system in accordance with the settings in the method to
be run. Before preparing the system, verify the settings in the Method Editor and make
sure that all accessories to be used are available.
WARNING
•
Do not use ÄKTA avant if it is not working properly, or if it has
suffered any damage, for example:
-
damage to the power cord or its plug
-
damage caused by dropping the equipment
-
damage caused by splashing liquid onto it
•
Always use appropriate Personal Protective Equipment (PPE)
during operation and maintenance of this product.
•
Do not use any accessories not supplied or recommended by
GE.
•
Fire Hazard. Before starting the system, make sure that there
is no leakage.
Checklist
Prepare the instrument and accessories according to the following information from
the Method Editor:
•
which valve ports to use for inlets and outlets
•
which column type to use
•
which column position to use
•
which buffers and samples to prepare
•
which sample application technique to use
•
make sure that the the pH electrode is connected, if applicable
•
which cassettes with corresponding deep well plates and/or tubes to use in the
fraction collector, if applicable
•
if it is a reversed phase chromatography (RPC) run, make sure to follow the safety
precautions below
104 ÄKTA avant Operating Instructions 29101556 AB
5 Prepare the system for a run
5.1 Before you prepare the system