5.6 Calibrate the pH monitor
Introduction
If pH will be measured during the chromatographic run, the pH monitor should be cali-
brated before the run is started. Use two pH calibration buffers with a difference of at
least one pH unit. Preferably use a pH standard buffer pH 4 or 7 as the first calibration
point, and a pH standard buffer close to the lowest or highest pH you need to measure
as your second point. Allow the buffers to reach the operating temperature before use.
Do not run a system flow during pH calibration.
Note:
Calibrate the pH monitor
CAUTION
pH electrode. Handle the pH electrode with care. The glass tip may
break and cause injury.
Follow the instructions to perform the calibration.
ActionStep
Open the System Control module. On the System menu, click Calibration.1
Result: The Calibration dialog box opens.
Select pH in the Monitor to calibrate list.2
Click Prepare for calibration.3
Result: The pH valve switches to the calibration position.
ÄKTA avant Operating Instructions 29101556 AB 119
5 Prepare the system for a run
5.6 Calibrate the pH monitor
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