ActionStep
•
For Pump P9: Connect the reference capillary Ref 2 (or an equivalent
capillary that gives a backpressure of 6 to 8 MPa [60 to 80 bar]) to one
of the column positions of the column valve (e.g., ports 1A and 1B).
•
For Pump P9H: Connect the reference capillary Ref 1 (or an equivalent
capillary that gives a backpressure of 2 to 3 MPa [20 to 30 bar] to one
of the column positions of the column valve (e.g., ports 1A and 1B).
3
Immerse the waste tubing in the buffer vessel to recirculate the liquid.4
•
If breaking in a pump piston seal of a system pump, follow the instruction
below:
In the Manual instructions dialog box:
-
Select Flow path:Column valve, and select the position of the capil-
lary connected to the column valve. Click Insert.
-
Select Flow path:Inlet A (for System Pump A) or Flow path:Inlet B
(for System Pump B) and select a Position. Click Insert.
-
Select Pumps and Pressures:Gradient and set Target to 0% B (for
System Pump A) or 100% B (for system pump B).
-
For Pump P9: Select Pumps and Pressures:System flow and set the
Flow rate to 5.0 ml/min. Click Insert.
For Pump P9H: Select Pumps and Pressures:System flow and set
the Flow rate to 25.0 ml/min. Click Insert.
-
Click Execute.
Result: A system flow starts.
•
If breaking in a pump piston seal of a sample pump, follow the instruction
below:
In the Manual instructions dialog box:
-
Select Flow path:Column valve, and select the position of the capil-
lary connected to the column valve. Click Insert.
-
Select Flow path:Sample inlet and select a Position. Click Insert.
-
Select Flow path:Injection valve and click Direct inject on the Posi-
tion menu. Click Insert.
-
Select Pumps and Pressures:Sample flow and set the Flow rate to
25.0 ml/min. Click Insert.
-
Click Execute.
Result: A sample flow starts.
5
ÄKTA avant Operating Instructions 29101556 AB 161
7 Maintenance
7.2 Replace pump piston seal, O-rings, and rinse membrane of Pump P9 or Pump P9H
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