ActionStep
Wipe clean the piston and remove all finger prints. Wet the piston with 20%
ethanol, and insert the piston into the return spring. With the pump head
facing downwards on the bench, insert the piston into the pump head by
pushing it gently but firmly vertically downwards into the seal.
2
Place the complete pump head over the locating pins on the front panel of
the sample pump module. Turn the pump head so that the text UP on the
drain plate is facing upwards. Push firmly on the front of the pump head,
and at the same time screw one of the screws to fasten the pump head
onto the front of the module using a hex wrench. Screw the second screw
of the pump head. Make sure to tighten both screws fully.
3
Reconnect the tubing of the pump piston rinsing system.4
Reconnect the inlet manifold.5
Reconnect the tubing to the pump head, and reconnect the pump inlet
tubing.
6
Break in the new pump piston seal, see instruction below.7
Break in the new pump piston
seal
Follow the instruction to break in the new pump piston seal of Pump P9-S.
ActionStep
Fill a buffer vessel with 20% ethanol in water. Immerse a piece of sample
inlet tubing, for example S1, in the buffer vessel. Place the buffer vessel on
the buffer tray.
1
Prime the inlets and purge the pump, see Section 4.5.2 Prime sample inlets
and purge Sample Pump, on page 93.
2
Connect the reference capillary Ref 1 (or an equivalent capillary that gives
a backpressure of 2 to 3 MPa [20 to 30 bar]) to one of the column positions
of the column valve (e.g., ports 1A and 1B).
3
Immerse the waste tubing in the buffer vessel to recirculate the liquid.4
ÄKTA avant Operating Instructions 29101556 AB 169
7 Maintenance
7.3 Replace pump piston seal, O-ring, and rinse membrane of Pump P9-S
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