B Reference information
34
Monitor UV-900 User Manual 18-1120-05 Edition AF
B Menus
B.1 Check menu
Checking autozero level
The instrument internal absorbance value for autozer o can be checked to test
the consistency in buffers.
1 Select main menu
Check
, press
OK
. The autozero absorbance value for
wavelength
1
is shown.
2 Turn the selection dial clockwise to display wavelengths
2
and
3
.
Lamp intensity, Lamp Ontime, Fliptime, UV Flow cell
See
Chapter
4
Maintenance.
Checking the wavelength
If there is any doubt that the instrument is showing the correct values, the
wavelength calibration can be checked.
1 Select main menu
Check
, press
OK
.
2 Select sub menu
Check
Wavelength
, press
OK
. The check st arts and after
approx. 20 seconds the message
Wavelength
OK
or
Wavelength
not
OK
is shown together with the deviation v alues. If not OK, re calibration can be
selected.
If a deviation in wavelength occurs repeatedly when checked, contact
Amersham Biosciences.
Recalibrate
If the instrument is left switched on for a long period (> 10 days), it may be
necessary to recalibrate it. This calibration is identical to that done when the
instrument is switched on.
1 Select main menu
Check
, press
OK
.
2 Select sub menu
Recalibrate
,
press
OK
. The recalibration starts and after
approx. 60 seconds the message
Recalibration
finished
is shown.
Check Autozero
AZ1 (215) 0.23456 AU
AZ2 (254) 0.23775 AU
AZ3 (280) 0.12326 AU
Checking Wavelength
Please wait...
Wavelengths OK
230+0 459+0 542+0 OK
Checking Wavelength
Please wait...
or
Wavelengths not OK
230-4 459+3 542+4 OK
Wavelengths not OK
Recalibrate? OK
Recalibrating
Please wait...
Recalibration
finished OK