E9Screen Software EPC9 Manual 39
AD-Channel: The oscilloscope can display the following:
• F2-Ext - External Stim. Input filtered by Filter 2
• Imon-1 - Current monitor 1
• Imon-2 - Current monitor 2
• Vmon - Voltage monitor output
• AD 0…5 - Any of the other AD channels.
The F2-Ext setting allows you to use Filter 2 as a general-purpose variable filter. In
this setting the External Stim Input becomes the filter input, and the filter output is
available at Filter 2 and is displayed in the Oscilloscope window.
Help: Displays keyboard equivalents that are assigned to EPC9 controls.
In this mode you can click on any control to have the Help window
display a short description of that control.
Sound: If this control is On, a sound is played with
its frequency coding for R-membrane. The sensitivity
(Hz/MΩ) and the volume can be specified. The
controls that set these parameters are normally
hidden, but may be accessed by closing the
Oscilloscope window.
V
0
(Pipette Offset): V
0
displays the offset
voltage (a voltage which is added to V-
membrane to obtain the pipette command voltage). It can be set either by the Auto-V
0
operation or by manually dragging the mouse after clicking into the item.
Furthermore, V
0
is changed automatically by the controlling program whenever the
user changes the variable LJ. This is necessary for LJ and the Auto-V
0
operation to
interact properly. It is not recommended that the user changes V
0
manually, because
this interferes with the software features for automatic offset correction.
Auto-V
0
: The Auto-V
0
button calls a procedure for automatic zeroing of the pipette
current. Thereby, an offset voltage (V
0
) to the pipette potential is systematically
varied until pipette current is zero. Range of V
0
is ± 200mV. Auto-V
0
is typically
performed before seal formation. It works properly only when a pipette is inserted
into the bath.
The Auto-V
0
procedure interacts with the variable LJ to provide for online correction
of liquid junction potentials and other offsets (see Chapter 7. Compensation
Procedures on page 59). This requires that V-membrane is set to the value of LJ (for On
Cell and In Out Recording Modes) or to the opposite polarity of LJ (for Whole Cell and
To Next Page
Loading...