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HEKA EPC 9 - Page 41

HEKA EPC 9
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E9Screen Software EPC9 Manual 41
Mode: Sets the Recording Mode (in the above example it is Voltage Clamp). For details
on the various recording modes see Chapter 6. Operating Modes on page 54.
Test - Sets the Test mode.
VC - Sets the Voltage Clamp mode.
CC - Sets the Current Clamp mode.
When switching from voltage-clamp to current-clamp mode, I-hold will be set to
inject the holding current required to keep the membrane voltage constant (“gentle
switch”, see description of the Gentle CC-Switch control below, subchapter Hidden
Controls). Thus, V-mon will be identical to the original V-membrane commanded
under voltage clamp. In analogy, upon returning to voltage clamp, V-membrane will
be set according to the membrane potential under current clamp and rounded to the
next 2 mV.
Quite often, the membrane potential under current clamp will
have changed, and when returning to voltage clamp, V-
membrane will possibly differ from that set before switching to current-clamp mode.
Last V-membrane can then be used to conveniently restore the original V-membrane
value. This button is normally hidden behind the oscilloscope window; it can be seen
when closing the oscilloscope. For details see Chapter 6. Operating Modes on page 54.
Starting with the version “C” of the EPC9, the current-clamp
circuitry has two speed settings (which can be toggled by a
button that is normally hidden behind the oscilloscope window; it can be seen when
closing the oscilloscope). For details see Chapter 6. Operating Modes on page 54.
Filter 1: Controls an analog 3-pole filter in
the current monitor pathway. The pop-up
menu provides for the following settings:
Bessel 100 kHz
Bessel 30 kHz
Bessel 10 kHz
HQ 30 kHz
Under most conditions a 10 kHz bandwidth is more than ample, and the filtering
reduces the high-frequency noise substantially. The HQ 30 kHz setting is selected
automatically when fast R
s
-compensation is in use; it is of little use otherwise.
Filter 2: Controls an analog 4-pole filter for
the current monitor 2 (I-mon 2). Filter 2 is in
series with Filter 1. Dragging the mouse or typing allows fine adjustment from 0.1-16

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