4.1.1 Calibrating the robotic needle arm 61
4.1.2 Calibration of the Single tube rack 63
4.1.3 Calibration of the MACSQuant X 5 Rack 63
4.1.4 Calibration of the 96-well plate 64
4.1.5 Calibration of the 384-well plate 64
4.1.6 Calibration of the MACSPlex Filter Plate 65
4.1.7 Calibration of custom 96 and custom 384 racks 65
4.1.8 Calibration of Universal Reagent Rack 65
4.2 Calibrating the rack detection 66
4.2.1 Single tube rack detection 66
4.3 Volume calibration 66
4.4 Tube length calibration 69
5 Hardware monitor 71
5.1 Fluidics 71
5.2 Sample uptake unit 71
5.3 Lasers and detectors 72
6 Live Support 73
7 Maintenance 75
7.1 Decontaminating the instrument 75
7.2 Exchanging fluid bottles 75
7.3 Exchanging hydrophobic air filters 75
7.4 Cleaning the washing station 75
7.5 Cleaning the uptake needle 76
7.6 Exchange the sample needle 76
7.7 Exchange the sample tubing 78
7.8 Exchange the needle sealing 81
8 Troubleshooting 85
8.1 Syringe pump leakage 85
8.2 Air bubbles during measurement / no event acquisition 85
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