Miltenyi Biotec MACSQuant X - Calibration of the Single Tube Rack; Calibration of the Macsquant X 5 Rack

4
CALIBRATION
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4.1.2 Calibration of the Single tube rack
1 Attach the Single tube rack with desired sample tube to the instrument as described in Install the Single
tube rack on page56.
2 Click Start calibration. The robotic needle arm will automatically move forward and insert the uptake
needle into the single tube. The uptake needle should be positioned at the center of the tube (i.e.
equidistant from the tube edges) almost touching the tube bottom (i.e. only a fraction of a millimeter from
the bottom of the tube). To check the uptake needle position, gently wiggle the tube to ensure that there is
a small amount of movement.
3 If this is not the case, carefully adjust the robotic needle arm accordingly.
4 Click Save calibration. The closed green circle indicates that the calibration is completed. Click Test to
confirm that the correct coordinates are saved.
4.1.3 Calibration of the MACSQuant X 5 Rack
For the correct usage of the MACS Orbital Shaker, please calibrate the MACSQuant X 5 Rack.
1 Attach the MACSQuant Orbital Shaker to the MACSQuant Instrument. Refer to the MACSQuant Orbital
Shaker user manual for details.
2 Click on the Tools tab on the sidebar of the Software and check the Calibrate uptake unit box. Red
closed circles indicate that the uptake components are not calibrated.
3 Click the Experiment tab on the sidebar of the MACSQuantify Software and select Chill 5 rack from the
Rack pull-down menu. If the MACSQuant X 5 Rack is not selected, the MACSQuant Instrument will display a
prompt screen.
4 Place the MACSQuant X 5 Rack onto the Orbital Shaker and place an empty 5 mL tube in position D6.
5 Click Start calibration. The robotic needle arm will automatically move forward and insert the uptake
needle into the tube in the position D6 of the rack. The uptake needle should be positioned at the center of
the tube (i.e. equidistant from the tube edges) almost touching the tube bottom (i.e. only a fraction of a
millimeter from the bottom of the tube). To check the uptake needle position, gently wiggle the tube to
ensure that there is a small amount of movement.
6 If this is not the case, carefully adjust the robotic needle arm accordingly.
7 Click Save calibration. The closed green circle indicates that the calibration is completed. Click Test to
confirm that the correct coordinates are saved. The MACSQuant Instrument will automatically test selected
positions.