Miltenyi Biotec MACSQuant X - Troubleshooting; Syringe Pump Leakage; Air Bubbles During Measurement; no Event Acquisition; Excessive Debris Is Present During Acquisition

8
TROUBLESHOOTING
85
8
Troubleshooting
8.1 Syringe pump leakage
Depending on the level of use, the syringe pumps should be cleaned every 1 to 3 months. Correct overnight
and long-term storage assures that no salt deposits accumulate in the syringe pumps, which could cause wear
of the pump seal. The syringe pump should not run dry at any time. This damages the pump seal. In case of
leakage of the syringe pump, turn off the instrument and check the connection between the syringe pump and
the valve. If it is loose, tighten it. If the leakage persists, contact Miltenyi Biotec Technical Support to replace
either the pump syringe or the pump seal.
8.2 Air bubbles during measurement / no event acquisition
Check for leakage within the fluidics and tubing system and perform a rinse program. Also, ensure that the
sheath particle filter is devoid of air. Ensure that the needle calibration was performed correctly. Furthermore,
check that the tubes connected to the bottle closures are tightly sealed. If the problem persists, contact Miltenyi
Biotec Technical Support.
8.3 Excessive debris is present during acquisition
1 Check all fluidics to ensure that all tubes are connected properly. Ensure that the sheath particle filter is
devoid of air.
2 Click the Rinse button in the Instrument status bar. Perform this twice.
3 If this does not work, try to clean the system by using the Clean and/or Flush program. To access the
Flush program, right-click on the Rinse button. Click the Clean button to perform a system clean with
1% hypochlorite solution. Follow the instructions in the dialog box that appears and place the required
bleach solution in the Single tube rack. The Single tube rack must be attached and selected in the
Experiment tab in order perform a Clean program. The Flush program will perform a system
backflush. This process will take approximately 16 minutes.
8.4 Touchscreen remains dark
If the touchscreen remains dark, check if the power cord is plugged in and the power supply is switched on. If
the instrument is switched on. If the instrument is switched on and the touchscreen remains dark, switch off the
instrument, wait five seconds and switch on again. If the instrument still does not initialize, contact Miltenyi
Biotec Technical Support.