Miltenyi Biotec MACSQuant X - Calibration of the 96-Well Plate; Calibration of the 384-Well Plate

4.1.4 Calibration of the 96-well plate
1 Click on the Tools tab on the sidepane of the Software and check the Calibrate uptake unit box. Red
closed circles indicate that the uptake components are not calibrated.
2 Click the Experiment tab in the sidepane of the Software and select the 96-well format from the Rack pull-
down menu. If the 96-well is not selected, the MACSQuant® Instrument will display a prompt screen.
3 Load an empty 96-well plate on the MACS® Orbital Shaker.
4 Click Start calibration. The robotic needle arm will automatically move forward and insert the uptake
needle into the well position H12 of the 96-well plate.
5 The uptake needle should be positioned at the center of the well (i.e. equidistant from the well edges)
almost touching the well bottom (i.e. only a fraction of a millimeter from the bottom of the well). To check
the uptake needle position, gently wiggle the plate to ensure that there is a small amount of movement.
6 If this is not the case, carefully adjust the robotic needle arm accordingly.
a) If the needle is on the left corner of the well, first push the MACS Orbital Shaker from the right, such that
the needle is on the right corner of the well. Then proceed with b)
b) If the needle is on the right corner of the well, push the MACSQuant Orbital Shaker from the left to reach
the center position of the needle in the well. The last movement of the MACS Orbital Shaker must be from
the left.
c) If the needle is on the rear corner of the well, first move the needle arm to the front, such that the needle
is on the front corner of the well. Then proceed with d)
d) If the needle is on the front corner of the well, move the needle arm to the front to reach the center
position of the needle in the well. The last movement of the needle arm must be from the front.
e) Push the needle down, almost touching the well bottom (i.e. only a fraction of a millimeter from the
bottom of the well). To check the uptake needle height, gently wiggle the plate to ensure that there is a
small amount of movement.
7 Click Save calibration. The closed green circle indicates that the calibration is completed. Click Test to
confirm that the correct coordinates are saved. The instrument will automatically test selected positions.
4.1.5 Calibration of the 384-well plate
1 Click on the Tools tab on the side pane of the Software and check the Calibrate uptake unit box. Red
closed circles indicate that the uptake components are not calibrated.
2 Click the Experiment tab on the sidebar of the Software and select the 384-well format from the Rack pull-
down menu. If the 384-well is not selected, the MACSQuant Instrument will display a prompt screen.
3 Load an empty 384-well plate onto the MACSQuant Orbital Shaker.
4 Click Start calibration. The robotic needle arm will automatically move forward and insert the uptake
needle into the well position H24 of the 384-well plate. The uptake needle should be positioned at the
center of the well (i.e. equidistant from the well edges) almost touching the well bottom (i.e. only a fraction
of a millimeter from the bottom of the well). To check the uptake needle position, gently wiggle the plate to
ensure that there is a small amount of movement.
5 If this is not the case, carefully adjust the robotic needle arm accordingly (refer to Calibration of the 96-
well plate above).
6 Click Save calibration. The closed green circle indicates that the calibration is completed. Click Test to
confirm that the correct coordinates are saved. The instrument will automatically test selected positions.
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CALIBRATION
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