Nikon ECLIPSE MA200 - Polarization Microscopy under the Episcopic Illumination (simplified;sensitive color)

Chapter 2 Microscopy
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2.3 Polarization Microscopy under the Episcopic Illumination (simplified/sensitive color)
Attach the items required for the polarization microscopy under the episcopic
illumination to the microscope. (See Table 2.1.)
Focus on the sample with the bright-field microscopy under the episcopic illumination.
(See Pages 16 to 17.)
Set the microscope for the polarization microscopy under the episcopic illumination.
1 Turn the revolving nosepiece to place the
objective of a desired magnification into the optical
path. (See 3.9.2.)
2 Push in the polarizer/analyzer unit to the second
click-stop position and place it into the optical
path. (See 3.13.1.)
3 Turn the polarizer rotation ring to the crossed
Nicol’s position. (See 3.13.2.)
4 To perform the sensitive color polarization
microscopy, insert the λplate into the
polarizer/analyzer unit and place it into the optical
path. (See 3.14.1.)
5 Turn the brightness control dial to adjust the
brightness of the episcopic illumination. (See
3.2.1.)
6 Adjust the brightness with the desired ND filter in
the filter turret. (See 3.10.)
7 Turn the polarizer rotation ring to adjust the
polarization while observing the image.
Return to the bright-field microscopy under the episcopic illumination.
1 Pull the analyzer/polarizer unit to the first
click-stop position to remove the
analyzer/polarizer from the optical path. (See
3.13.1.)
2 Both the λplate and the polarizer/analyzer unit are
removed from the optical path. (See 3.14.1.)
3 Turn the brightness control dial to adjust the
brightness of the episcopic illumination. (See
3.2.1.)
4 Adjust the brightness with the desired ND filter in
the filter turret. (See 3.10.)
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