Nikon ECLIPSE MA200 - Dark-field Microscopy under the Episcopic Illumination

Chapter 2 Microscopy
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2.2 Dark-field Microscopy under the Episcopic Illumination
Attach the items required for the dark-field microscopy under the episcopic illumination
to the microscope. (See Table 2.1.)
Focus on the sample with the bright-field microscopy under the episcopic illumination.
(See Pages 16 and 17.)
Set the microscope for the dark-field microscopy under the episcopic illumination.
1 Turn the revolving nosepiece to place the
objective of a desired magnification into the optical
path. (See 3.9.2.)
2 Pull out the BD field changeover lever to select the
“DF (dark-field)” position. (See 3.3.)
The aperture diaphragm and the field diaphragm
are fully opened. (The dial positions do not
change.)
When the polarizer/analyzer or the fl filter is
placed into the optical path, it is automatically
excluded.
3 Turn the brightness control dial to adjust the
brightness of the episcopic illumination. (See
3.2.1.)
4 Adjust the brightness with the desired ND filter in
the filter turret. (See 3.10.)
Return to the bright-field microscopy under the episcopic illumination.
1 Push in the BD field changeover lever to select the
“BF (bright-field)” position. (See 3.3.)
The aperture diaphragm and the field diaphragm
automatically return to the previous positions.
(The dial positions do not change.)
2 Turn the brightness control dial to adjust the
brightness of the episcopic illumination. (See
3.2.1)
3 Adjust the brightness with the desired ND filter in
the filter turret. (See 3.10.)
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