Chapter 2 Microscopy
28
2.7
Polarization Microscopy under the Diascopic Illumination (simplified/sensitive color)
Attach the items required for the polarization microscopy under the diascopic
illumination to the microscope. (See Table 2.1.)
Focus on the sample with the bright-field microscopy under the diascopic illumination
and center the condenser. (See pages 23 to 26.)
Set the microscope for the polarization microscopy under the diascopic illumination.
1 Turn the revolving nosepiece to place the
objective of a desired magnification into the optical
path. (See 3.9.2.)
2 Push the polarizer/analyzer unit attached to the
MA200 main body into the optical path. (See
3.13.1.)
When using the sextuple revolving nosepiece,
insert the D-DA analyzer slider into the slot on the
revolving nosepiece instead of the
polarizer/analyzer unit, if desired. (See 3.17.1.)
3 Place the T-P2 polarizer into the optical path and
make a crossed Nicol’s position. (See 3.23.8.)
4 To perform the sensitive color polarization
microscopy, place the TI-DIC λplate into the
optical path. (See 3.23.8.)
When using the sextuple revolving nosepiece, use
the D-LP λplate instead of the TI-DIC λplate, if
desired. (See 3.18.1.)
5 Turn the light intensity control dial on the TI-PS
100W power supply to adjust the brightness of the
diascopic illumination.
6 Push in or pull out the desired ND field for the
supporting pillar for dia-Illuminator to place it into
the optical path, then adjust the brightness for the
view-field. (See 3.23.5.)
Return to the bright-field microscopy under the diascopic illumination.
1 Remove the analyzer from the optical path.
2 Remove both the T-P2 polarizer and the λplate
from the optical path.
3 Turn the light intensity control dial on the TI-PS
100W power supply to adjust the brightness of the
diascopic illumination.
4 Push in or pull out the desired ND field for the
supporting pillar for dia-Illuminator to place it into
the optical path, then adjust the brightness for the
view-field.
3-6
3-5
3-3, 4
3-2
3-1
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