Chapter 2 Microscopy
27
Center the condenser. (See 3.23.2.)
1 Be sure that the objective is set to the 10X
objective.
If not, turn the revolving nosepiece to place the 10X
objective into optical path.
2 Stop down the field diaphragm by turning the field
diaphragm dial on the supporting pillar for
dia-Illuminator until the field diaphragm image
comes into the view-field.
3 Turn the condenser focus knob for the supporting
pillar for dia-Illuminator to focus on the field
diaphragm image.
4 Turn the two condenser centering screws for the
pillar illuminator to move the field diaphragm
images to the center of the view-field.
5 Turn the revolving nosepiece to place the 40X
objective into the optical path.
6 Turn the field diaphragm dial for the supporting
pillar for dia-Illuminator until the diaphragm image
becomes nearly the same as the view-field.
7 Turn the two condenser centering screws on the
supporting pillar for dia-Illuminator to move the field
diaphragm images to the center of the view-field.
Set the desired magnification and observe the sample.
1 Turn the revolving nosepiece to place the objective
of a desired magnification into the optical path.
2 Move the aperture diaphragm lever on the system
condenser to adjust the aperture diaphragm to a
size of 70 to 80% of the objective N.A. (See
3.23.4.)
3 Turn the field diaphragm dial for the supporting
pillar for dia-Illuminator until the diaphragm
becomes nearly the same as the view-field. (See
3.23.3.)
4 Push in or pull out the desired ND filter on the
supporting pillar for dia-Illuminator to place it into
the optical path, then adjust the brightness for the
view-field. (See 3.23.5.)
If accurate color reproduction is not crucial (e.g., for
color photography), change the lamp voltage with
the light intensity control dial on the TI-PS100W
power supply.
5 Look into the eyepieces. Move the stage to bring
the observation target into the center of the
view-field.
6 Turn the coarse/fine focus knobs and focus on the
sample.
Note: Condenser refocusing clamp (See 3.23.6.)
To raise or lower the condenser, secure the clamp in advance. You can restore the condenser position
easily.
7-2, 6
7-3
7-1, 5
7-4, 7
8-4
8-3
8-2
8-4
8-6
8-1
8-5
8-5
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