Chapter 2 Microscopy
18
Set the desired magnification and observe the sample.
1 Turn the revolving nosepiece to place the
objective of a desired magnification into the optical
path. (See 3.9.2.)
2 Turn the coarse/fine focus knobs and refocus on
the sample. (See 3.7.)
3 Turn the brightness control dial to adjust the
brightness of the episcopic illumination. (See
3.2.1.)
4 Turn the field diaphragm dial so that the field
diaphragm image circumscribes the view-field.
(See 3.11.)
5 Turn the aperture diaphragm dial so that the
aperture diaphragm image becomes 70 to 80% of
the numerical aperture of the objective. (See
3.12.)
6 Adjust the brightness with the desired ND filter in
the filter turret. (See 3.10.)
Note: Adjusting focus
It may be difficult to focus on a sample with small contrast, such on a polished surface. In a case like
this, stop down the field diaphragm so that its image can be seen in the view-field, and try to focus on
the frame of the diaphragm image. When the frame is in focus, the sample is in focus just as well.
5-2
5-3
5-6
5-5
5-4
5-1
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