Chapter 2 Microscopy Operations
59
Differential
Interference Contrast
Microscop
Preparation
Focus and Optical
System Adjustment
Microscopy
Differential Interference
Contrast Microscopy
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15
Bring an arbitrary DIC objective into the optical path.
Press the OBJ CW or CCW button to bring the desired
objective into the optical path.
Oil immersion
When using an oil immersion type objective, apply Nikon
specified non-fluorescence immersion oil between the
specimen and the objective. (See Chapter 3 “14
Oil/Water Immersion”.)
Never apply oil between the universal condenser top
lens and the specimen. The universal condenser is
designed for dry use.
Note that an oil/water immersion type objective cannot
be used for the motorized universal condenser.
Motorized nosepiece toggling
Two objectives can be alternated by specifying an
objective pair. Objectives can be switched using the
[OBJ CW/CCW] button on the microscope. (See
Chapter 3 “11.1 Motorized Nosepiece Operation”.)
Bringing desired DIC objective into optical path
PA_ 40x____Z:__1234.567um
OPEN__AS30.0mm__FS30.0mm
[NOSEPIECE] button, sub screen: Desired objective
16
Attach the DIC slider (on the objective) to the nosepiece.
Select a DIC slider suited for the objective and the
microscopy purpose, and then insert it into the slot
(immediately above the objective) on the nosepiece.
(See Chapter 3 “17 Differential Interference Contrast
Microscopy” - “17.2 Using Optical Elements”.)
1
2
A
λ
10x
Bring objective side DIC slider into optical path
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