NOVA BIOMEDICAL BIOPROFILE FLEX2 - System Details; ESM15 Vs. ESM250; ESM System Details

2-1
2 System Details
2. System Details
2 System Details
2.1 ESM15 vs. ESM250
The ESM15 serves to integrate the FLEX2 with the ambr® 15, and the ESM250 serves
to integrate the FLEX2 with the ambr® 250 automated bioreactor system. In either
conguration, the components, set up, operation, and maintenance required for the
External Sampling Module are largely the same, with a few key differences. When
pertinent, sections in this manual will use "ESM15" or "ESM250" notation to distinguish
between different sets of guidelines. In sections where the External Sampling Module
is referred to generally as "ESM," one can assume the guidelines are shared between
both the ESM15 and ESM250 congurations.
2.2 ESM System Details
All ow components and consumables critical to ESM operation are shared between the
ESM15 and ESM250. The key difference lies in the ESM software, which is customized
for the integrated uid handling system (i.e. ambr® 15 or ambr® 250). The External
Sampling Module consists of the components listed below and pictured in Figures 2.1
and 2.2.
○ A multi-port syringe pump that draws the sample from the ESM sample cup to
the sample port inside the BioProle FLEX2 Analytical Unit (AU) and controls the
ow of ESM reagents from the ESM Reagent Cartridge.
○ An External Tubing Set (replaceable by the End User) for delivery of sample
material from the ESM sample cup to the FLEX2, supply of System Fluid solution
and Depro solution from the reagent cartridge to the ESM sample cup for ow
path cleaning, and evacuation of residual sample waste.
○ A Reagent Cartridge Bay with a needle shroud manifold and internal tubing for
supply of reagents from the ESM Reagent Cartridge used for ow path priming
and cleaning, and for evacuation of waste.
○ A COM port for communication between the ESM and FLEX2 and control of
hardware components.
○ An AD/Valve port for control of ESM ow components.
○ A power entry module for independent power supply to the ESM.
Syringe
Pump
Reagent Pack
Bay
Figure 2.1 ESM Front View
Power Entry
Module
COM Port
AD/Valve
Port
Figure 2.2 ESM Rear View