NOVA BIOMEDICAL BIOPROFILE FLEX2 - ESM Reagent Cartridge Replacement

4-2
BioProfile FLEX2 ESM Instructions for Use Manual
4.1.1 ESM Reagent Cartridge Replacement
Step 1: Septum and Pinch Valve Tubing Installation
A sample port septum and pinch valve tubing must be installed in the BioProle FLEX2 for
the ESM sample to be properly aspirated by the FLEX2 sample probe. A new septum and
pinch valve tubing segment are included with each new ESM Reagent Cartridge. Nova
Biomedical recommends that these components be installed with each ESM Reagent
Cartridge replacement or after extended periods of ESM inactivity.
To install/replace septum:
1. Open the FLEX2 main door and locate the ESM
sample port inside the FLEX2 Analytical Unit. The
sample port is situated on the ESM top plate, in
front of the clear, wash/waste well station.
2. Remove the septum cap (black upper portion of
the sample port) from the lower amber portion by
turning it counterclockwise to expose the septum.
3. Remove the old septum and install a new
one.
4. Thread the cap of the sample port back on by
turning clockwise until it is nger tight. Do not
over-tighten.
CAUTION: Do not use pliers or a wrench when
tightening the sample port. Over-
tightening of the threaded portion may
crack or break the assembly.
To install/replace pinch valve tubing:
1. Locate the white ESM pinch valve inside the FLEX2
Analytical Unit. The pinch valve is located to the
right of the ESM Sample Port.
2. Remove the white tubing from the pinch valve
and carefully disconnect the ends of the tubing
from each port.
3. Connect each end of the new tubing to the open ports and seat the tubing fully
inside the pinch valve.
WARNING: Cell culture samples are potential sources of infectious agents. Handle
all sample and ow path components with care. Gloves and protective
clothing are recommended.
NOTE: If the 1/4"-28 barbed tting swivel adapter (the tting to which the right end
of the pinch tubing is connected) becomes damaged during pinch tubing
replacement, Nova offers a Swivel Adapter Replacement Kit (PN 61326).
Sample Port
Assembly
Sample Port Cap
Removed & Septum
Installed
Sample Port
Cap & Septum
Removed
Pinch Tubing
Figure 4.3 ESM Sample Port Assembly