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Rayto RT-7600S - Blood Sample Handling

Rayto RT-7600S
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RT-7600S Auto Hematology Analyzer User’s Manual
15
Blank test only shows test results of WBC, RBC, HGB, HCT and PLT. The operator can run
blank test at any time required. Press “Aspiration Key” to start blank test. During test, the
indicator light turns red, status column on top of screen will display test status.
Blank test acceptable range is as follows:
Parameter Reference Range
WBC 0.2 × 10
9
/ L
RBC 0.02 × 10
12
/ L
HGB 1 g / L
HCT 0.5 %
PLT 10 × 10
9
/ L
If the results of blank test are not in this acceptable range, please repeat the steps above
until the results are good. If the results are still out of range after 5 times’ tests, please
check if the reagent and connections of pipeline are good. And please try to use functions
like “Remove Blockage”, “Back Flush”, “Cleaning” and “Concentrated Cleanser Soaking” in
the software Service Menu to solve possible aperture blockage problem.
3.3 Blood sample collection
Blood sample collection is classified as venous blood and peripheral blood.
Warning: do not touch blood sample, QC sample and calibration sample
directly. Handling of these objects shall comply with related
operation instruction.
3.3.1 Venous blood collection
Venous blood can be collected by using vacuum negative pressure tube or ordinary method
under atmospheric pressure, anticoagulant must be dropped to all venous blood collection
containers in advance, usually EDTA.K
2
.2H
2
O is adopted as anticoagulant, with content of
1.5-2.2mg/ml.
3.3.2 Peripheral blood collection
Blood sampling position:
For adults, the inner side of middle finger or ring finger tips of left hand is better; for
children above half a year old, middle finger is better; for infant below half a year old,
blood is usually sampled from thumb or outer side of foot bottom.
Blood sampling method:
It shall be carried out in accordance with peripheral blood collection standard of health
authority. Peripheral blood collection normally adopts local centesis; the typical collection
method is to pierce through finger end. Blood tube uses 20μL constant volume blood
tube or bullet blood tube. It is recommended to collect no less than 30μL blood to
facilitate double check.
During blood sampling, if blood flow stagnates, one may slightly press farther end of
wound, never force around piercing hole. Avoid tissue fluid mixed with blood, hampering
test analysis result accuracy.
3.3.3 Blood sample mixing
Before test, blood sample must be thoroughly shaken and mixed, the recommended method
is: shake up and down, rotate test tube 3-5min, do not shake too violently.
Blood sample to be tested can only stored at room temperature, test shall be done in 4h,

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