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2) Select velocity
Test the impedance corresponding to the median velocity of 4 cycles and 5 cycles, and
compares it with the target impedance, and selects the median velocity which the
impedance is closer to the target impedance for adjustment.
The impedance values in this manual are obtained when both the internal and external fluid
of the microelectrode are Ringer's solutions.
Example: the impedance corresponding to the median velocity of 30 for 5 cycles is 2M, and
the impedance corresponding to the median velocity of 40 for 4 cycles is 6M, while the
target impedance is 3±1M; the impedance corresponding to the median velocity for 5
cycles is more close to the target impedance, so select velocity 30; if the target impedance is
5M, select velocity 40.
3) Adjust parameters based on the selected velocity
The cycle value should remain unchanged during parameter adjustment.
Single-line adjustment method
Adjust the velocity or heat until the microelectrode pulled meets the needs. The velocity
adjustment range is ±10, and the heat adjustment range is ±30. Heat should be adjusted
first.
Figure 4-16
Multi-line adjustment method
Use multi-line adjustment method if the target microelectrode cannot be pulled by the
single-line adjustment or the cycle value changes after the single-line adjustment. The heat
adjustment range is ±30, and the velocity adjustment range is ±10.
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