5.2
Fundamentals
of
Operation
I
Note
I
When the liquid inside the flow lines is not sufficiently replaced
with the mobile phase, the baseline takes longer to become stabilized
and the
drift
becomes large.
To
perform effective replacement, turn
R
flow
ON/OFF
several times at an interval of
two
minutes.
Switching flow lines during solvent delivery at large flow rate
in mode
L
may damage the solenoid valve and the flow cell.
Thus, the following message appears when performing
R
flow
in mode
L.
f
CHECK
FLOW
7
Press
[E)
after the flow rate is changed to
1
mumin.
To
avoid trouble caused by bubbles, refer to the following.
When the pump sucks bubbles, degas the solvent with the
ultrasonic cleaner. If dirt on
the suction filter is the cause,
clean the filter with the ultrasonic cleaner or replace it.
Bubbles may not be easily removed when using
an
aqueous
solvent. Flush the flow lines with methanol
or
acetone.
When replacing the aqueous solvent with organic solvent or
vice versa, bubbles may be generated successively. If
bubbles can be observed, flush the flow lines with thoroughly
degassed solvent.
5.
Setting the measurement mode
(Example)
When changing the measurement mode from
A
to
P
Press
[shift)
and
.
The mode is changed to
P
to perform optical balance
adjustment.
balance
RI
(lo)
range
mode
100
BALANCE
P
0
0
4
P
5
-
7
RID
-
1OA
-
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