6.1
System Performance Check
(8)
Press
[ZERO
I,
Is)
,
lo)
and
[
Enter
1
on the Chromatopac to
center
the
Den Position.
(9)
Press mid
on
the ChromatoDac to dot the
output.
(10) Wait
till
the
baseline becomes stable.
Good
example
0
Stable
Bad
examples
x-
There are
spi kings.
Increases
with
the
X
.-H
lapse of
time.
'-\
Decreases with the
lapse
of time.
'X
Unstable
(11) Set
AmN
to
8
on the Chromatopac. (Chromatopac full
scale becomes equivalent to about
256
x
10-6RIU)
(12)
Press
(5)
,
,
-
and (=)on
the
Chromatopac.
plotting.
(14) Inject sample to the injector.
(15)
Press [=]of the Chromatopac simultaneously with the
sample injection.
4.
Example
of
analysis
result
An example
of
analysis under the following conditions
is
shown
below.
Mobile phase
:
AcetonitrilelWater
=
75/25
Column
:
Asahipak
NH2P-50
4.6mm
0
x
25cm
Flow rate
:
1
mwmin
Column temp
:
40°C.
Sample
:
Fructose
in
loomL
water,
lo&
Grucose 100 mg injection
Sucrose 100 mg
Maltose
100 mg
Raffmose
100 mg
RID
-
1OA
-
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