F
-
polarity
mode
Span adjustment should be performed over each mode of
A,
P
and
L.
When a Chromatopac is used, refer to
8.2.1,
and when
an
LC
workstation is used, refer to
8.2.2.
Inject
the
standard solution whose refractive index is
known,
and
adjust the detector output
so
that the signal intensity can be verfied.
Following
is
the description for mode
A
span adjustment.
+
A
(When performing mode A
span
adjustment)
I
Items to be prepared
I
Qty
I
Chromatopac
or
LC
workstation
Sy-ringe
.
Adapter
Water
(HPLC
grade)
lmL
I
Refractiveindex standardsample*
I
lOOmL
I
*
Refractive index standard sample:
glycerin solution 0.872
gL
Dissolve 43.6 g of glycerin
(USP
grade or equivalent)
in
the
1L
of
water, and dilute this solution
50
times for the refractive index
standard solution. (Reference document
ASTM
E1303-89) This
sample shows difference of
100
x
10
"
RIU
from that
of
pure water.
8.2.1
When span adjustment
is
performed
using the Chromatopac
(1)
Turn
ON
the power.
(2)
Set the parameters of the detector
as
follows:
I
L
ILOCAL I1
(*I
I
m
E
Q)
E
c
-
*
When the
RID-
1
OA
is
controlled from the system controller
SCL
-
1
OA:
This
adjustment is performed by the detector independently. First, input
LOCAL
=
1
to
separate control
(link)
from the
SCL.
After that, input
each
set
value using the numerical keys of the detector
main
unit
8
-
3
RID
-
1OA
-
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