Pos: 102 /100 Sig ma/110 BA Ze ntrifugen Sigma (Proj ekte)/1-1 4_1-14K/061 Betrieb/060-00 20-0020-0020 Einsetzen des Mi krohämatokrittellers 1-14_ 1-14K @ 28\mod _140532081217 6_68.docx @ 195489 @ 4 @ 1
6.2.2.2 Installation of the microhaematocrit rotor
Fig. 5: Microhaematokrit rotor (part no. 11026) with reader (part no. 17028)
• Unscrew the rotor fastening nut from the motor shaft (anti-clockwise).
• Lower the microhaematocrit rotor with its central bore straight down
onto the motor shaft.
• Hand-tighten the rotor fastening nut clockwise (corresponds to 1 Nm).
The rotor fastening nut and conical spring washer must face the
microhematocrite rotor (i.e. the writing must be shown on top).
Fig. 6: Rotor fastening nut from above (A) and from below (B)
• Follow the safety instructions and hazard warnings (see chapter 3 -
"Safety")!
Operation
• Fill the capillary tubes with blood and seal them at one end with putty or
by fusion.
• Place the capillary tubes into the recesses of the rotor with the sealed
end against the rubber ring. Ensure that the capillary tubes fit tightly
against the rubber ring. The opposite places must be loaded.
• Put the rotor cover on and lock it.
• Close the centrifuge lid.
• Enter the following parameters: speed 13,000 rpm, gravitational field
RCF max. 11,903 x g, time approx. 5 min.
• Start the centrifuge.
• Open the centrifuge lid when the rotor has stopped.
• Unlock the rotor cover and lift it off.