Process
Page 20 of 60 M1393R0 OQ/PQ Operating Instructions
3.2.2 B FAMOS Microautosampler
To prepare the FAMOS Microautosampler for the OQ/PQ tests:
a) Position the caffeine standards of the OQ/PQ kit in the FAMOS sample rack as
presented in TABLE 3-5.
TABLE 3-5 Sample Positions in the Autosampler Rack
Tray Position Sample
A2
0.25 µg/ml Caffeine (CAP system only)
A3
0.5 µg/ml Caffeine (CAP system only)
A4
1.0 µg/ml Caffeine
A5
2.5 µg/ml Caffeine
A6
5.0 µg/ml Caffeine
A7
10.0 µg/ml Caffeine
A8
20.0 µg/ml Caffeine
B1
40.0 µg/ml Caffeine (NAN system only)
B2
60.0 µg/ml Caffeine (NAN system only)
b) Use mobile phase A as the wash solvent (TABLE 3-4).
c) Degas the wash solvent. Helium sparging is strongly recommended.
d) Check that there is no air in the syringe and run a wash cycle on the instrument.
3.2.2 C Switchos Advanced Microcolumn Switching Unit
To prepare the Switchos Advanced Microcolumn Switching Unit for the OQ/PQ:
a) Prepare the Switchos with the following mobile phases
Solvent A: 0.1 % TFA in water
Solvent B: 0.1 % TFA in water
Solvent C: 0.1 % TFA in water
Solvent D: 0.1 % TFA in water.
b) Connect a trap-column connecting tubing 30 µm ID x 15 cm (P/N 160182) to port 2 of
switching valve A.
c) Start helium sparging to degas the solvents.
d) Thoroughly purge all channels as described in the user’s manual of the instrument.
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