B-2 BioMate 3 Operator’s ManualThermo Electron Corporation B-2
Appendix B - Parameters
Factor 3-31 Abs(WL3-31) x Factor = Result
Parameter Description
ID# Numeric Identifier - automatically increments during test until parameter is
reset or test is exited
Intercept Where line crosses the y-axis (abs where conc=0)
Interval Time Time between repeated readings
Language Language of any text on screen
Linearity Value To help determine the linearity of the reaction during the measurement, the
spectrophotometer offers a linearity parameter. This parameter is the
difference between the changes in absorbance of two measurements as
shown in the following example:
Time____ Abs___ ∆Α__ Line___ arity_____
1 .1 ---- ----
2 .2 .1 ----
3 .29 .09 P
4 .38 .09 P
5 .46 .08 P
6 .52 .06 F
Linearity is the ∆A between ∆A calculations.
P = Pass F = Fail
Lock/Unlock Used to protect stored tests from accidental deletion or alteration
Low/High Limits Lowest & highest acceptable results, outside of which the result is flagged
as 'Low' or 'High'
Measurement Mode The type of photometric data reported for a measurement
Molarity of cation Molarity of Na
+
in incubation mixture
Number of Bases Number of bases in the oligonucleotide
Number of Matched Number of cuvettes that will be run in the Correction Program (maximum of
Cuvettes 5)
Number of Samples Number of samples to be measure in the whole test
Number of Standards Number of standards to be measured for standard curve
Phot Accy Tolerance ±Photometric accuracy acceptable
Pk-Pk Noise Tolerance ±Noise acceptable
Printer Selects output mode
Recorder Chart High/Low Range (high or low) setting for chart recorder
Ref. Wavelength Internal Reference wavelength; for each reported measurement, measures
analytical wavelength & reference wavelength.
Reported measurement = abs @ analytical WL - abs @ reference WL
Ref. Wavelength Turns internal zeroing on or off
Correction
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