Thermo BioMate 3 series User Manual

Appendix C - Calculations
Thermo Electron Corporation C-4
Calculation Entry Parameters Formula Displayed Units
Calculation of Tm: • # units A, # units T, T
m
= 78°C + 16.6log ((Na
+
)/ °C
RNA-RNA hybrids # units G, # units C (1+0.7(Na
+
)) + 0.7(%GC) –
• M = molarity of cation 500/L – P – 0.35(%formamide)
• Fraction GC = fraction
of G and C
• %form = %formamide
in the sample
• L = # of base pairs
• P = % mismatching
Conversion from • µg/mL and molecular pmol/µL= µg/mL x 1000 pmol/µL
weight from Oligo (calc DNA Mol. Wt.
factor) test

Thermo BioMate 3 series Specifications

General

Brand	Thermo
Model	BioMate 3 series
Category	Measuring Instruments
Language	English