Warning: Observe Good Laboratory Practice (GLP) at all times, particularly when
working with hazardous materials. Consult the Material Safety Data Sheets regarding
the solvents you use. Additionally, consult the safety representative for your
organization regarding its protocols for handling such materials.
Warning: To avoid personal contamination with biologically hazardous or toxic compounds,
wear clean, chemical-resistant, powder-free gloves when performing this procedure.
2.5.1
Installation recommendations for fittings
Three types of fittings connect the 30-cm CH or 30-cm CHC to the system: PEEK (polymer-
based) fittings and tubing, SST (stainless steel) tubing and fittings, and for Arc Bio applications,
MP35N (a nickel-cobalt-chromium-molybdenum alloy) tubing. When connecting tubing, heed the
following recommendations for installing and tightening fittings.
Recommendations:
• To prevent band spreading, ensure that the tubing bottoms in its fitting before you tighten the
compression screw.
• For easier accessibility, use compression fittings with long flats to attach tubes to the column-
selection valve.
• Whenever you loosen fittings during maintenance, examine them for cracks, stripped threads,
and deformations.
• Do not reuse stainless steel fittings more than six times.
2.5.1.1
Assembling new fittings
For metallic (SST or MP35N) fitting and tubing assemblies with ferrules not previously assembled
or set to tubing, you must mark the compression screw and fitting body, and ensure that the two
marks line up when you tighten them.
Requirement: Wear clean, chemical-resistant, powder-free gloves when performing
this procedure.
Requirement: Use eye protection when performing this procedure.
Required tools and materials
• 1/4-inch open-end wrench – For tightening or loosening stainless steel (gold-plated) fittings
with 2-piece ferrule.
• Permanent marker
December 5, 2017, 715004751 Rev. C
Page 38
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