Xylem YSI 2500 - L-Lactate

7-3
7.2.2 L-Lactate
This is a direct reading of L-Lactate (L-Lactic Acid) in solution at the enzyme sensor. The enzyme L-Lactate Oxidase is
immobilized in the enzyme membrane.
L-Lactate + O
2 L-Lac Oxidase
H
2
O
2
+ Pyruvate
System Buffer
YSI 2357
Calibrator Std
YSI 2776
Linearity Std
YSI 1530
Membrane
YSI 2329
Membrane
Color
Gray
Detection
Range
0.05–2.70 g/L
Calibration
Point
0.5 g/L
Linearity Check
Point
2.67 g/L
Sample Size
25 µL
End Point
30 sec
Precision
(CV,n=10)
2% or 0.03 g/L,
whichever is
greater
Linearity
±2% or 0.03 g/L,
whichever is
greater
(0.05 to Cal Point)
±5%
(Above Cal Point to
Range Max)
Typical Working
Life
14 days
Note: See Appendix B – Concentration
Unit Conversion if concentration unit
conversion is required.
Special Considerations:
• If sample dilution is required, use reagent water.
• D-Lactate is not a substrate for L-Lactate Oxidase.
Therefore, the 2500 Analyzer cannot directly measure
D-Lactate. If you have a known racemic mixture of
lactates, the L-Lactate value multiplied by 2 should give
you the total lactate value.