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Zeiss Auto Focus - Troubleshooting

Zeiss Auto Focus
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MAINTENANCE, REPLACEMENT OF FUSES AND SERVICE
Auto Focus equipment Troubleshooting Carl Zeiss
06/2013 425131-7044-001 33
5.3 Troubleshooting
General checklist for error situations and non-standard events:
Has installation or cabling work been correctly performed (see section 3 on page 19)?
Check the cabling of the Auto Focus.
Are cable ends firmly seated in their sockets?
Was the Switching on procedure duly followed (see section 4.1 on page 22)?
Is the beam splitter of the camera deflection in the beam path?
Is the correct camera deflection installed?
Are the cables of the Auto Focus under mechanical strain? Check the cables and ensure that they are
free from strain.
Have the objectives been calibrated? Repeat or renew the focal position calibration.
Is the active objective correctly displayed in the TFT display?
Has a sample actually been inserted and a typical region on the sample been selected?
Shift to a different XY position of the sample. Pronounced irregularities / scratches on the sample may
result in disruptive reflections at the current sample position.
Does the sample have structures which run parallel to the Auto Focus stripe structures? Reposition the
sample or, if this is not possible, select a different angle setting for the Auto Focus sensor.
Which type of sample has been inserted? It may be necessary to change the surface of specimen
setting (Reflective / Partially reflective / Diffuse).
Is the stage Z-position far outside of the capture range of the objective? Use a lower objective
magnification for refocusing or make a rough focus manually.
Is a sample with a cover glass being used? Only EC Epiplan objectives (with magnification of 10x or
less) and EC Epiplan-Neofluar objectives (with magnification of 10x or less) from the list of compatible
objectives can be used for samples with a cover slip.

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