MAINTENANCE, REPLACEMENT OF FUSES AND SERVICE
Auto Focus equipment Troubleshooting Carl Zeiss
06/2013 425131-7044-001 33
5.3 Troubleshooting
General checklist for error situations and non-standard events:
• Has installation or cabling work been correctly performed (see section 3 on page 19)?
Check the cabling of the Auto Focus.
• Are cable ends firmly seated in their sockets?
• Was the Switching on procedure duly followed (see section 4.1 on page 22)?
• Is the beam splitter of the camera deflection in the beam path?
• Is the correct camera deflection installed?
• Are the cables of the Auto Focus under mechanical strain? Check the cables and ensure that they are
free from strain.
• Have the objectives been calibrated? Repeat or renew the focal position calibration.
• Is the active objective correctly displayed in the TFT display?
• Has a sample actually been inserted and a typical region on the sample been selected?
Shift to a different XY position of the sample. Pronounced irregularities / scratches on the sample may
result in disruptive reflections at the current sample position.
• Does the sample have structures which run parallel to the Auto Focus stripe structures? Reposition the
sample or, if this is not possible, select a different angle setting for the Auto Focus sensor.
• Which type of sample has been inserted? It may be necessary to change the surface of specimen
setting (Reflective / Partially reflective / Diffuse).
• Is the stage Z-position far outside of the capture range of the objective? Use a lower objective
magnification for refocusing or make a rough focus manually.
• Is a sample with a cover glass being used? Only EC Epiplan objectives (with magnification of 10x or
less) and EC Epiplan-Neofluar objectives (with magnification of 10x or less) from the list of compatible
objectives can be used for samples with a cover slip.