OPERATION
Axio Observer Illumination and contrast techniques ZEISS
12/2016 431004-7244-001 167
The values for a and b (see Fig. 160) are
determined using the eyepiece reticle or the
micrometer eyepiece.
If you are working with white light (without
interference filter), λ = 550 nm must be used.
When using interference filters, their center
wavelength applies.
The measured path difference is aperture-
dependent and decreases with increasing
illumination aperture.
Accordingly, the following correction values must be taken into account depending on the objective
used:
Objective Correction factor k
5x/0.15 1.0057
10x/0.25 1.0161
10x/0.30 1.0236
20x/0.4 1.0436
20x/0.50 and 50x/0.75 1.0718
50x/0.60 1.1111
50x/0.75 and 100x/0.75 1.2038
50x/0.80 1.2500
50x/0.90 and 100x/0.90 1.3929
100x/0.95 1.5241
Table 1: Aperture-dependent correction
Example:
a = 11 mm b = 5 mm
λ = 550 nm Objective 20x/0.50
nm134
mm22
0718.1mm5nm550
a2
kb
d =
⋅⋅
=
⋅⋅λ
=
Fig. 160 Interference fringe pattern
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