Agilent Technologies 1260 Infinity G1315C - Page 102

102 Agilent 1260 Infinity DAD and MWD User Manual
5 How to optimize the Detector
Optimization Overview
Figure 32 Choosing a Flow Cell in HPLC
5 Setting the slit width
• Use 4 nm slit for normal applications.
• Use narrow slit (e.g 1 nm) if your analytes have narrow absorbance bands
and for high concentrations.
• Use a wide slit (e.g. 16 nm) to detect very low concentrations.
• Optimizing spectral acquisition (DAD only)
• Select spectra acquisition mode according to your needs (see Tab l e 13 on
page 87).
• Set the spectral wavelength range (for colorless samples 190...400 nm is
sufficient).
• Set step to 4 nm for normal use; set small step (and slit width) if high
resolution of spectra with fine structure is wanted.
• spectral resolution, sensitivity and
linearity.
Tabl e 1 5 Optimization Overview
Parameter Impact
FP
FP
PLQ
PLQ
PLQ
PLQ
,QWHUQDOFROXPQGLDPHWHU PP PP PP PP
+LJKSUHVVXUH
IORZFHOOIRU
SUHVVXUHV
DERYHEDU
7\SLFDOFROXPQ
OHQJWK
7\SLFDOSHDN
ZLGWK
5HFRPPHQGHGIORZFHOO
7 FP
! FP
7\SLFDOIORZ
UDWH

POPLQ

POPLQ

POPLQ

POPLQ

POPLQ
6WDQGDUG
IORZFHOO
6HPLPLFUR
IORZFHOO
0LFURRU
6HPLQDQR