Agilent 1260 Infinity DAD and MWD User Manual 115
How to optimize the Detector
5
Optimizing Selectivity
Ratio Qualifiers for Selective Detection of Compound Classes
Ratio qualifiers can be used where, in a complex sample, only one particular
class needs to be analyzed — a parent drug and its metabolites in a biological
sample, for example. Another example is the selective analysis of derivatives
after pre- or post-column derivatization. Specifying a signal ratio that is
typical for the sample class is one way of selectively plotting only those peaks
that are of interest. The signal output remains at zero so long as the ratio is out
of the user-specified ratio range. When the ratio falls within the range, the
signal output corresponds to the normal absorbance, giving single, clear peaks
on a flat baseline. An example is shown in Figure 42 on page 115 and
Figure 43 on page 116.
Figure 42 Wavelength Selection for Ratio Qualifiers
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