Agilent Technologies Agilent 1260 Infinity - Page 31

1260 Infinity Binary LC - System User Guide 31
Introduction
2
Theory of Using Smaller Particles in Liquid Chromatography
A chromatographic separation can be optimized based on physical
parameters of the HPLC column such as particle size, pore size,
morphology of the particles, the length and diameter of the column, the
solvent velocity, and the temperature. In addition, the thermodynamics of
a separation can be considered and the properties of the solute and the
stationary and mobile phases (percentage of organic solvent, ion strength,
and pH) can be manipulated to achieve the shortest possible retention and
highest selectivity.
Figure 8 Selecting optimal conditions for HPLC
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