32 1260 Infinity Binary LC - System User Guide
2 Introduction
Theory of Using Smaller Particles in Liquid Chromatography
Resolution can be described as a function of three parameters:
• column efficiency or theoretical plates (N),
• selectivity (α),
• retention factor (k).
According to the resolution equation ( Figure 9 on page 32 ), the
selectivity has the biggest impact on resolution (Figure 10 on page 32).
This means that the selection of appropriate mobile and stationary phase
properties and temperature is critical in achieving a successful separation.
Figure 9 Resolution equation
Figure 10 Effect of plate number, separation factor and retention factor on R
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