1260 Infinity Binary LC - System User Guide 57
Optimization of the Agilent 1260 Infinity Binary LC
3
How to Achieve Higher Resolution
Extra-column Volume
To maintain the high resolution achieved on the column, the extra- column
volume, especially after the column, should be as low as possible.
• For 4.6 mm inner diameter columns the standard delay configuration
should be used for the pump, see Figure 15 on page 45.
• For 2.1 mm inner diameter columns, the low delay configuration should
be used for the pump, and the low dispersion kit should be installed in
the column compartment. For highest UV sensitivity, the short mixer is
also recommended. See Figure 13 on page 43 and Figure 14 on
page 44.
• The injection volume is also of importance, especially if the sample is
dissolved in an organic solvent. In this case, the gradient should start
with a low percentage of organic phase to focus the compounds at the
top of the column. This avoids peaks dispersion due to the injection.
• The 10 mm Max- Light cartridge cell has a low dispersion volume (σ
volume 1.0 µL) and no further volume optimization is required.
• In situations where the alternative 60 mm Max- Light high sensitivity
cell is used to get higher sensitivity the cell volume is optimized for the
use with 3 mm and 4.6 mm inner diameter columns.
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