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Agilent Technologies Agilent 1260 Infinity - Page 90

Agilent Technologies Agilent 1260 Infinity
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90 1260 Infinity Binary LC - System User Guide
5 Quick Start Guide
Data Acquisition in Method and Run Control View
Checkout specifications:
four peaks are visible in the chromatogram
Intensity Test – pass
Wavelength Calibration±1nm
Tabl e 1 0 Method parameters for first separation run - low delay volume configuration
Module Parameter Setting
Pump Solvent A Water
Solvent B Acetonitrile
Flow rate 0.75 mL/min
Solvent
Composition
40 % A, 60 % B
Stop Time 1.2 min (1 min post time)
Autosampler Injection 1 µL
Column
Compartment
Column Column: SB-C18, 4.6x 50 mm, 1.8 µm, 600 bar
(827975-902)
Te m p er a t u re 4 0 ° C
Detector Signal Wavelength: 250 nm, Bandwidth: 4 nm
Reference Wavelength: 360 nm, Reference
Bandwidth: 100 nm

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