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EVOS
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User Guide
REV B Published 20 SEP 2010
ADVANCED OPERATION
USING THE QUICKSAVE OPTION
QuickSave allows you to save multiple images under a
single base file name. Simply specify the settings and select
the QuickSave option (in the Overlay tab), and EVOS will
save each image with a single click of the Save button.
1. Click the Settings button to open the Settings
dialog box, and then select the QuickSave tab.
2. Click in the Base Filename text field and enter a name
that describes the imaging session. The orange “Next”
file name will reflect the information entered.
3. Click in the Count text field to enter the starting
number, if you do not want to start at 1. The orange
“Next” file name will reflect the information entered.
4. Select a file format (.tif or .png) from the File Type
drop-down menu .
5. Click the Browse button to select a destination folder
for the QuickSave files. In the Browse popup, highlight
the desired folder and click OK .
6. To create a new folder, first click the name of the desired
parent folder, and then click the New Folder button .
Enter a folder name and date, if desired. After creating
the new folder, click OK to close the Browse popup.
Note: Clicking the Date button anywhere within a
text field will automatically insert the current
date (MM-DD-YYYY) wherever the cursor is in
that field.
7. Select “Also save each channel separately”
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to save
multiple channels for each image. This will create up to
five files per captured image, named according to the
following conventions:
BaseName_RGB_0001.tif (Overlay image)
BaseName_channel_0001.tif (where “channel” is the
selected channel, such as GFP, RFP, DAPI, or TRANS)
When optional LED light cubes are installed, the files
will automatically save channels with their names. See
Changing LED Light Cubes (p.23) for more information.
8. Click OK
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to accept QuickSave settings.
9. Select the Overlay tab and click the radio button for the
QuickSave option
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.
Note: The color camera version displays the QuickSave
option in all tabs.
10. After acquiring an image with the Capture button, click
Save
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. The image will be saved as specified in the
QuickSave settings.
Virtual keyboard
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Settings: QuickSave Tab
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QuickSave option radio button
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Settings button and Save button
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Browse popup (to select a destination folder)
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