www.amgmicro.com 32
EVOS
fl
User Guide
REV B Published 20 SEP 2010
PROBLEM POSSIBLE SOLUTIONS
Misaligned overlay image Re-capture images in each channel.
Transmitted light image is too dim
(at higher magnifications)
Set the phase annuli selector to the BF position.
Remove condenser slider, if one is in place.
Remove light shield box, if it is in place.
Specks, dots, or blurs on image
Follow instructions under Objective Lens Care (p.31) to clean objectives.
Uneven focus across screen
Position specimen flat on the stage; be sure the specimen’s thickness is even.
Trouble focusing on coverslipped
specimen on standard slide
Place the slide so the coverslip is facing up. (Long working-distance objectives require a
thick optical substrate, and image best through 1.0 - 1.5 mm of glass or plastic.)
LCD screen is black
Click the LIGHT ON button (onscreen).
Move objective selection wheel so that light shines through objective.
Verify that the phase annuli selector on condenser is not stuck between settings.
Center specimen over objective.
Verify power supply is connected and power switch is on.
LCD screen is red, or red patches
cover parts of the screen
Dim the illumination until the red highlights disappear to get the maximum level of
brightness without any overexposed areas.
Disable the “Highlight saturated pixels in red” option in the Settings (see p. 27).
IMAGE QUALITY ISSUES
PROBLEM POSSIBLE SOLUTIONS
Image does not respond to
changes in focus or stage position
Click the LIGHT ON button. (Note that a red USB icon on the Save button indicates there is an
unsaved image, which will be lost unless it is saved before clicking LIGHT ON.)
LIGHT ON/OFF button is inactive
Verify that the light cube selection lever is clicked into a position.
Verify that the objective selection wheel is clicked into a position.
Scalebar does not appear when
clicked
Capture image first; scalebar is only available after capturing an image.
Save button does not respond
when clicked
Click capture first; It is only possible to save an image that is captured. The USB icon on the
Save button is red when there is a captured image to save.
DVI output does not work on an
external LCD monitor or projector
Power EVOS o , check the cable connections, and power on the microscope.
Verify that the external display accepts DVI-D input (i.e. digital input). A monitor with
analog input only (DVI-A) will not display the EVOS’ digital output.
Unable to connect to network
Verify physical cable connections; confirm the Ethernet jack is active.
Use a compatible network adaptor (see p. 21 for a list of supported adapters).
If the physical connections are good, and the problem persists, contact your network
administrator to resolve any network issues. Note that a Linux network must use Samba for
EVOS to be able to find it.
SOFTWARE INTERFACE ISSUES
TROUBLESHOOTING
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