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EVOS
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User Guide
REV B Published 20 SEP 2010
This section describes all the onscreen and mechanical
controls in detail. To see the most commonly used controls
in context, refer to the QUICK-REFERENCE DIAGRAMS (p.8).
ONSCREEN CONTROLS
This glossary is not alphabetized. Onscreen items are listed
from top to bottom first, and then from left to right.
CHANNEL INDICATOR BAR
The channel indicator bar (top) highlights whichever
one of the following light channels is currently
selected:
Lever position 1 (Transmitted in example; closest to
front of microscope)
Lever position 2 (GFP in example; second from front)
Lever position 3 (RFP in example; second from back)
Lever position 4 (DAPI in example; closest to back)
See also Light Cube Selection Lever (p.30).
LOGIN BUTTON
The Login button (AMG logo, bottom left) allows for
logging in and creating or changing user profiles. This
button also displays the current user profile.
CONTROL BAR
The control bar (bottom left) varies depending on
which tab is selected. Choose a tab by clicking on it in
the left end of the control bar:
Find & Focus: to avoid photobleaching while setting
up a fluorescence specimen
Actual: to view image using actual acquisition
parameters (LED brightness and exposure time)
Overlay: to view multiple fluorescence channels; also,
the QuickSave option is available on this tab.
FIND & FOCUS TAB
Use the Find & Focus tab to view the sample with
either transmitted light (to minimize photobleaching)
or fluorescence, and to acquire images. This feature
displays 10 frames/second for focusing and captures
images at longer-exposure, high-quality settings. The
following controls are available in the Find & Focus tab:
LIGHT ON/OFF button
Illumination slider
Image capture button
Color option
Boost option
Channel indicator bar with GFP channel highlighted
Control bar variations: Find & Focus, Actual & Overlay
Find & Focus tab
Login button (set to Guest profile)
Note: In all tabs, the color camera version displays the
QuickSave option instead of the Info bar.
CONTROLS GLOSSARY
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