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EVOS
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User Guide
REV B Published 20 SEP 2010
CONTROLS GLOSSARY
ACTUAL TAB
Use the Actual tab with fluorescence channels to view
the image at the actual exposure time used for high-
quality image capture. With Actual tab selected, EVOS
responds more slowly to stage position and focus
changes, depending on the user-selected exposure time
for the camera. The following controls are available in
the Actual tab:
LIGHT ON/OFF button
Illumination slider
Exposure time slider
Image capture button
Color option
Boost option
OVERLAY TAB
Use the Overlay tab to select and overlay multiple
fluorescence channels as a single multicolor image.
The following controls are available in the Overlay tab:
Brightness and Contrast sliders for each channel
Boost option for each channel
QuickSave option (to the right, not shown)
VIEW: SCALEBAR OPTION
The Scalebar option (bottom center) is a toggle
button that displays or hides the Scalebar tool. This
option is only available after an image is captured. To
move the Scalebar, simply click and drag it.
VIEW: TOOLBAR OPTION
The Toolbar option (bottom center) includes the Time
Lapse, Transfection, Count, and Image Review tools. Click
the small gray triangle to open each tool.
TIME LAPSE TOOL
The Time Lapse tool allows you to set up the interval/
duration and the file name/destination for time lapse
sessions. See Recording Time Lapse Images (p.16).
TRANSFECTION TOOL
The Transfection tool automatically captures and
overlays images for transfection analysis. See Using
the Transfection Tool (p.17).
COUNT TOOL
The Count tool allows you to tag cells and other
features using up to 6 labels, and it calculates the
totals and percentages for each label. See Counting
Cells (p.18).
Actual tab
Overlay tab
Scalebar
View: Scalebar
and Toolbar
options
Time Lapse tool
Transfection tool
Count tool
Toolbar
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