27. Cleaning and Disinfectant
Disinfecting the incubator interior
The incubator should be cleaned and disinfected with substances that can be removed with proper
rinsing. Any toxic substance or its residue could be biologically toxic which could potentially affect the
culture conditions and specimen development. The instructions here included have demonstrated to
clean and disinfect the incubator and its components allowing proper culture conditions as demonstrated
by validation testing and Mouse Embryo Assay (MEA).
Preparation
It is recommended to use gloves, safety goggles as well as a protective coat. Make sure the incubator is
unplugged.
Procedure
1. Turn the incubator off and unplug the power cord from the wall outlet. Shut off the CO
2
supply from
the gas cylinder or gas manifold.
2. Open the lid of the incubation drawers and remove water from the slot.
3. Remove the aluminum incubation block out of the drawers.
4. Wipe all incubation drawer surfaces (internal), lids with a 3% solution of 7X detergent in distilled
water using a damp sponge or a soft cloth (Kim wipes).
5. Rinse with distilled water using a damp sponge or soft cloth.
6. Wipe all incubator surfaces (internal), lids with OH Peroxide solution (3%).
7. Rinse with distilled water using a damp sponge or soft cloth.
8. Dry the incubator chamber out with a lint-free soft cloth.
9. Wrap the aluminum incubation blocks for sterilization (use steam sterilizer or oven only - DO NOT
USE ETYLHENE OXIDE METHOD for this purpose).
10. Once the sterilization of the aluminum incubation block is completed and the parts are at room
temperature open the wrapping and install it back in the drawers.
11. Plug in the incubator and turn the incubator on. Pour humidity-water to the water slot with water at
room temperature (or higher temperature) for humidification of the drawers.
12. Initiate Set Up Mode. The incubator will start heating until it reaches the set temperature. The
chamber will increase its humidity gradually. Open the CO
2
flow from the gas cylinder or manifold.
The incubator will not inject CO
2
until the Set Up Mode cycle is completed.
13. After Set Up Mode is completed (12 hours) the incubator will initiate CO
2
injection into the chamber
to a pre set 5%. Adjust the incubator to the desired setting if different than the factory setting (5%
CO
2
and 37C).
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