Washer Problems| 99
Problem Possible Cause What To Do
Loose covers on waste
bottles.
Firmly tighten waste bottle covers.
Dispense rate too fast
for volume selected.
Specify slower dispense Flow Rate or lower
volume.
Faulty vacuum pump. Contact BioTek TAC.
Insufficient or no
vacuum.
Firmly seat the waste bottle covers.
Check all external tubing for kinks or clogs.
When the program begins, you should be
able to hear the vacuum pump turn on. If it
is not turning on, contact BioTek TAC.
If the vacuum pump turns on, remove the
vacuum tubing from the back of the
instrument and put your finger over the port.
If there is no vacuum, contact BioTek TAC.
Uneven
dispensing
of fluid;
wells not
filled.
Clogged dispense tubes
on the washer manifold.
Remove and clean the washer manifold
Manifold or tubing not
adequately primed.
Run W-DAY_RINSE once or twice.
Dispense flow rate too
low. Flow rate 1 or 2 CW
is used with 384-well
plates.
Define a higher dispense Flow Rate.
Microplate aspiration
height adjustment too
high or too low.
Change the aspirate height (Z-axis position)
in the protocol.
BioTek Instruments, Inc.
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