38 | 405 TS Getting Started Guide
405 Recommendations for Priming the Washer
These recommended prime volumes are required to achieve 95% or higher purity
when changing wash buffers.
Model Buffer Switching One Buffer
405 TS/LS 300 250
HT 300 250
Buffer Switching models have four buffer valves; otherwise, only one reagent bottle
may be connected to the washer. For Select models (with Cell Wash (CW)
capability), the recommended prime volume is the same with and without Buffer
Switching.
Model Quick Prime Prime step
Select 300 Main Low Flow
150 150
Manifold Prime:50 mL is recommended to clear air from the manifold only and
to wet the tips. This is recommended before running a protocol to correct fluid loss
due to evaporation.
Dead Volume
BioTek's recommended prime volumes are based on purity testing and measured
dead volumes. Generally, priming with three times the dead volume assures
purity.
Model Dead Volume
405 TS/LS 108 mL
HT 108 mL
Select 129 mL
For models with buffer switching tubing, dead volume is ± 2 mL additionally.
405™ TS Microplate Washer
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