Predefined Sample Protocols| 43
W-Corning_
FLAT
Standard wash protocols modified to best position the manifold
tubes for dispensing and aspirating to Corning Costar flat-
bottomed and round-bottomed wells.
W-Corning_
ROUND
W-NUNC_
384
Standard wash protocols modified to best position the manifold
tubes for dispensing and aspirating to Nunc
®
flat-bottomed
wells, round-bottomed wells, and 384-well plates.
W-NUNC_
FLAT
W-NUNC_
ROUND
Bead Assays - Biomagnetic Separation and Bottom Filtration
W-Luminex_
MAG_plate_
96
Biomagnetic separation wash protocols designed for optimal bead
recovery in 96-well round- or flat-bottomed plates and 384-well
plates when using BioTek's Flat magnets. Begins by soaking the
plate for 1 minute to let the beads settle at the bottom of the
wells, then performs a 2 cycle wash with 30 second soaks
between cycles.
Recommendation: Determine Magnet Height Offset on page 61
and apply it for the plate you are using, especially when using
384-well plates.
W-Luminex_
MAG_384
W-Luminex_
VAC_96
Vacuum filtration wash protocols for Luminex
®
xMap polystyrene
bead assays using filter-bottom plates, 96- and 384-well. Protocol
begins with a 5 second bottom aspiration, followed by a 200 µL
dispense for 96-well plates and 75 µL dispense for 384-well, and a
two cycle 200/75 µL wash.
W-Luminex_
VAC_384
Wash Step Parameters Table
Minimally, a wash step includes an aspirate step followed by a dispense step. Select
and define each option to customize the parameters for your assay.
Option Description/Values range
Default
values
Number of
wash
cycles:
Each wash cycle first aspirates and then dispenses
fluid to and from the plate.
3
BioTek Instruments, Inc.
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